Recombinant Human IFN- Omega protein

SKU: CD01607

Size:20 ug

Background:Interferon-Omega (IFN-ω) coded by IFNW1 gene in human, is a number of the type I interferon family, which includes IFN-α, IFN-β, and IFN-ω. The IFNAR-1/IFNAR-2 receptor complex can help with the signal transduction, followed the antiviral or the antiproliferative actions. IFN-ω is derived from IFN-α/β and share 75 % sequence with IFN-α. It has two intramolecular disulfide bonds which are crucial for activities. Mire-Sluis et al have described bioassays for IFN-α, IFN-β, and IFN-ω that exploit the ability of these factors to inhibit proliferation of TF-1 cells induced by GM-CSF. The bioassays can be used also with Epo and TF-1 cells, or Epo and Epo-transfected UT-7 cells.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interferon-Omega

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a chemotaxis bioassay using human TF-1 cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 18 IU/mg.

Synonyms:IFN-ω, IFNW1MHuman

Endotoxin:Less than 1 EU/μg of rHuIFN-ω as determined by LAL method.

Storage:-20ºC

Sequence:CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS

uniprot accession: P5

Molecular weight:Approximately 2. kDa, containing 172 amino acid residues with two conserved disulfide bonds.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.