Recombinant Human G-CSF protein

SKU: CD01736

Size:2 ug

Background:Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition it may function in some adhesion or recognition events at the cell surface. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30 a.a. signal peptide and have identical amino acid sequences except for a three a.a. insertion (deletion) at the 35th a.a. residue from the N-terminus of the mature protein. Human G-CSF is 73 % identical at the amino acid level to Mouse G-CSF and the two proteins show species cross-reactivity.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Granulocyte Colony Stimulating Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse NFS-6 cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:G-CSF, CSF3,GCSF; CSF3OS; C17orf33,Human

Endotoxin:Less than 1 EU/μg of rHuG-CSF as determined by LAL method.

Storage:-20ºC

Sequence:TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

uniprot accession:P9919

Molecular weight:Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1mM sodium acetate buffer, containing 5 % trehalose, pH 4..