Recombinant Human Friend leukemia integration 1 transcription factor (FLI1)

SKU: Q01543

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Cancer

Uniprot ID: Q01543

Gene Names: FLI1

Alternative Name(s): Friend leukemia integration 1 transcription factor(Proto-oncogene Fli-1)(Transcription factor ERGB)

Abbreviation: Recombinant Human FLI1 protein

Organism: Homo sapiens (Human)

Source: E.coli

Expression Region: 1-452aa

Protein Length: Full Length

Tag Info: N-terminal 10xHis-tagged

Target Protein Sequence: MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQPHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY

MW: 57.0 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Sequence-specific transcriptional activator (PubMed: 24100448, PubMed: 26316623, PubMed: 28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Reference: "Macrothrombocytopenia and dense granule deficiency associated with FLI1 variants: ultrastructural and pathogenic features." Saultier P., Vidal L., Canault M., Bernot D., Falaise C., Pouymayou C., Bordet J.C., Saut N., Rostan A., Baccini V., Peiretti F., Favier M., Lucca P., Deleuze J.F., Olaso R., Boland A., Morange P.E., Gachet C. Alessi M.C. Haematologica 102: 1006-1016(2017)

Function: