Recombinant Human FGF-23 protein

SKU: CD01802

Size:5 ug

Background:Human FGF-23 belongs to the FGF-19 subfamily which has three members FGF-19, 21, 23. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. They are classically considered to be paracrine factors and are known for their roles in tissue patterning and organogenesis during embryogenesis. By contrast, the FGF-19 subfamily has recently been shown to function in an endocrine manner. Members of this subfamily have poor ability of binding to heparin binding site which is a crucial factor in ligand-receptor complex formation. β-Klotho has been identified as co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. FGF-23 is most highly expressed in bone, from which it circulates through the blood to regulate vitamin D and phosphate metabolism in kidney.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Fibroblast Growth Factor-23

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than .5 μg/ml, corresponding to a specific activity of > 2. × 13 IU/mg in the presence of .3 μg/ml of rMuKlotho and 1 μg/ml of heparin.

Synonyms:FGF-23, FGF23,ADHR, FGFN, HFTC2, HPDR2, HYPF, PHPTC,Human

Endotoxin:Less than 1 EU/μg of rHuFGF-23 as determined by LAL method.

Storage:-20ºC

Sequence:YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI

uniprot accession:Q9GZV9

Molecular weight:Approximately 25.3 kDa, a single non-glycosylated polypeptide chain containing 227 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.