Recombinant Human FGF-2/FGFb Protein

SKU: PKSH032438

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: FGF-2;FGFb

Target Synonym: Basic fibroblast growth factor;Bfgf;FGF-2;FGF2;FGFB;Fibroblast growth factor 2;HBGF-2;Heparin-binding growth factor 2

Research Areas: Signal Transduction;Cardiovascular;Neuroscience;Cancer;Developmental Biology;Stem cells

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: N-His

UNIProt ID: P09038

Accession: P09038

Background: Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.

Concentration:

Activity: Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human FGF-2 is approximately >5 x 10⁵ IU/mg.

Sequence: Ala 135-Ser 288

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS with 0.01% sarkosyl,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.1 kDa

ObservedMW: 17 kDa