Recombinant Human ENO1 protein (GST tag)

SKU: PDEH101092

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:ENO1

Target Synonym:2 phospho D glycerate hydro lyase;Alpha enolase;C myc promoter binding protein;ENO1L1;Enolase 1 (alpha);MBP 1;NNE;PPH;Tau crystallin

Research Areas:Signal Transduction;Epigenetics and Nuclear Signaling;Cancer;Metabolism;Carbohydrate metabolism;

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:P06733

Accession:P06733

Background:Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Concentration:

Activity:Not validated for activity

Sequence:Ser 2-Lys 434)

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:47.5 kDa

ObservedMW:60 kDa