Recombinant Human eIF4E protein (GST,His tag)

SKU: PDEH101060

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:eIF4E

Target Synonym:EIF4E;EIF4EL1;EIF4F;Eukaryotic translation initiation factor 4E;eIF-4E;eIF-4F 25 kDa subunit;mRNA cap-binding protein

Research Areas:Epigenetics and Nuclear signaling

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST & C-His

UNIProt ID:P06730

Accession:P06730

Background:Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Concentration:

Activity:Not validated for activity

Sequence:Ala 2-Val 217

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:48.7 kDa

ObservedMW:52 kDa