Recombinant Human DCAMKL1 Protein (aa 1-705, His & GST Tag)

SKU: PKSH030359

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CL1;CLICK1;DCAMKL1;DCDC3A;DCLK

Research Areas: Neuroscience

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion tag: N-His-GST

UNIProt ID: O15075

Accession: O15075-1

Background: DCAMKL1; also known as DCLK1; is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains; which bind microtubules and regulate microtubule polymerization; a C-terminal serine/threonine protein kinase domain; which shows substantial homology to Ca2+/calmodulin-dependent protein kinase; and a serine/proline-rich domain in between the doublecortin and the protein kinase domains; which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes; including neuronal migration; retrograde transport; neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

Concentration:

Activity: The specific activity was determined to be 6. 1 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.

Sequence: Met 1-Val 705

Purity: > 84 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 0.5mM PMSF

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 106 kDa

ObservedMW: 105 kDa