Recombinant Human CD28 Protein(hIgG1 Fc Tag)

SKU: PDMH100448

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Immune Checkpoint Proteins

Abbreviation:CD28

Target Synonym:PAS;T-cell-specific surface glycoprotein CD;CD28;Tp44;CD28 antigen;CD28 molecule;GPSAT;GYPA;HGpMiX;MNS;PAS2;T-cell-specific surface glycoprotein CD28;T44

Research Areas:Immunology;Signal Transduction;Cancer

Conjugation:

Target Species:Human

Expression Host:Mammalian

Application:

Fusion tag:C-hIgG1 Fc

UNIProt ID:P10747-1

Accession:P10747-1

Background:CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Concentration:

Activity:Not validated for activity

Sequence:Asn19-Pro152

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method

Calculated MW:39.6 kDa

ObservedMW:55-70 kDa