Recombinant Human CD16a/FCGR3A Protein (176 Phe, His&AVI Tag), Biotinylated

SKU: PKSH030284

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CD16;CD16A;CD16a;CD16a Antigen;FCG3;FCGR3;FCGR3A;Fc-Gamma RIII;Fc-Gamma RIII-Alpha;Fc-gamma RIIIa;FcR-10;FcRIII;FcRIIIa;IGFR3;IgG Fc Receptor III-2;Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A

Research Areas: Immunology,Stem Cells

Conjugation: Biotinylated

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His-Avi

UNIProt ID: P08637

Accession: P08637-1

Background: The Fc receptor with low affinity for IgG (FCGR3; or CD16) is encoded by 2 nearly identical genes; FCGR3A and FCGR3B; resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A; it is also known as CD16a; encodes a transmembrane protein expressed on activated monocytes/macrophages; natural killer (NK) cells; and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells; independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells; independent of antibody ligation.

Concentration:

Activity: Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a. The EC50 of Human CD16a is 2. 4 - 5. 7 μg/ml. 3. Labeling ratio of biotin to protein: 0.7-1.

Sequence: Met 1-Gln 208

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.1 kDa

ObservedMW: 48 kDa