Recombinant Human CD122/IL-2RB Protein

SKU: PKSH031478

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: CD122/IL-2RB

Target Synonym: P70;IL2RB;CD122;IL15RB;P70-75;IL-2Rβ;p75;IL-2RB;Interleukin-2 receptor subunit beta;IL-2 receptor subunit beta;IL-2R subunit beta;High affinity IL-2 receptor subunit beta;Interleukin-15 receptor subunit beta;p70-75 (p75);IL-2RβCD122 antigen;High affinity IL 2 receptor beta subunit;High affinity IL 2 receptor subunit beta;IL2 receptor;Interleukin 2 receptor beta;interleukin 2 receptor subunit beta;P70 75;P7075

Research Areas: Signal Transduction;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture;ELISA

Fusion tag: None

UNIProt ID: P14784

Accession: NP_000869.1

Background: Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

Concentration:

Activity: 1. Immobilized human IL2Rb at 10 μg/ml (100 μl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg/mL. 2. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15.The ED50 for this effect is 1-4μg/mL.

Sequence: Met 1-Asp 239

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.2 kDa

ObservedMW: 40 kDa