Recombinant Human CASK Kinase Protein

SKU: PKSH030972

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CAGH39;CAMGUK;CMG;FGS4;LIN2;MICPCH;MRXSNA;TNRC8

Research Areas: Neuroscience,Signal Transduction

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: None

UNIProt ID: O14936

Accession: O14936-4

Background: Peripheral plasma membrane protein CASK; also known as calcium/calmodulin-dependent serine protein kinase; CASK and LIN2; is a nucleus; cytoplasm and cell membrane protein which belongs to theMAGUK family. CASK / LIN2 contains oneguanylate kinase-like domain; twoL27 domains; onePDZ (DHR) domain; oneprotein kinase domain and oneSH3 domain. CASK / LIN2 is ubiquitously expressed. Expression of CASK / LIN2 is significantly greater in brain relative to kidney; lung; and liver and in fetal brain and kidney relative to lung and liver. CASK / LIN2 is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. CASK / LIN2 contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. It binds to cell-surface proteins; including amyloid precursor protein; neurexins and syndecans. CASK / LIN2 may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Defects in CASK are also the cause of FG syndrome type 4 which is an X-linked disorder characterized by mental retardation; relative macrocephaly; hypotonia and constipation.

Concentration:

Activity: Not validated for activity

Sequence: Ala 2-Tyr 898

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 102.1 kDa

ObservedMW: 102 kDa