Recombinant Human CA9 Protein( Fc Tag)

SKU: PDMH100227

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Immune Checkpoint Proteins

Abbreviation:CA9

Target Synonym:CA-IX;CA9;CAH9;CAIX;Carbonate dehydratase IX;Carbonic anhydrase 9;Carbonic anhydrase IX;Carbonic dehydratase;G250;Membrane antigen MN;MN;P54/58N;pMW1;RCC associated protein G250;RCC-associated antigen G250;Renal cell carcinoma-associated antigen G250

Research Areas:Cancer;Cardiovascular;Metabolism;Signal Transduction

Conjugation:

Target Species:Human

Expression Host:Mammalian

Application:

Fusion tag:C-Fc

UNIProt ID:Q16790

Accession:Q16790

Background:Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Concentration:

Activity:Not validated for activity

Sequence:Gln38-Asp414

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:66.3 kDa

ObservedMW:80 kDa