Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial

SKU: O60271

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Developmental Biology

Uniprot ID: O60271

Gene Names: SPAG9

Alternative Name(s): JIP-4;JNK-interacting protein 4;Cancer/testis antigen 89;CT89;Human lung cancer oncogene 6 protein;HLC-6;JNK-associated leucine-zipper protein;JLP;Mitogen-activated protein kinase 8-interacting protein 4;Proliferation-inducing protein 6;Protein highly expressed in testis;PHET;Sperm surface protein;Sperm-associated antigen 9;Sperm-specific protein;Sunday driver 1

Abbreviation: Recombinant Human SPAG9 protein, partial

Organism: Homo sapiens (Human)

Source: Baculovirus

Expression Region: 408-534aa

Protein Length: Partial

Tag Info: C-terminal 6xHis-tagged

Target Protein Sequence: REVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEM

MW: 15.8 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex. Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking.

Reference:

Function: