Recombinant Human APN Protein(Fc Tag)

SKU: PDMH100347

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:APN

Target Synonym:LAP;ANPEP;APN;CD13;GP150;LAP1;P150;PEPN;Aminopeptidase N;AP-M;AP-N;CD13 antigen;CD13APN;PEPNhAPN; gp150;Alanyl (membrane) aminopeptidase;Alanyl aminopeptidase;Aminopeptidase M;AMPN;AP M;AP N;CD 13;hAPN;LAP 1;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13

Research Areas:Immunology;Stem Cells

Conjugation:

Target Species:Human

Expression Host:Mammalian

Application:

Fusion tag:C-Fc

UNIProt ID:P15144

Accession:P15144

Background:Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

Concentration:

Activity:Not validated for activity

Sequence:Lys69-Lys967

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:123.8 kDa

ObservedMW:120-130 kDa