Recombinant Human ACP1/LMW-PTP Protein (GST Tag)

SKU: PKSH031337

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: ACP1;Adipocyte Acid Phosphatase;HAAP;LMW-PTP;LMW-PTPase;Low Molecular Weight Cytosolic Acid Phosphatase;Low Molecular Weight Phosphotyrosine Protein Phosphatase;Red Cell Acid Phosphatase 1

Research Areas: Cell Biology,Cancer

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Enzyme

Fusion tag: N-GST

UNIProt ID: P24666

Accession: AAI06012.1

Background: The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.

Concentration:

Activity: Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is > 65, 000 pmol/min/μg.

Sequence: Met 1-His 158

Purity: > 88 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 44.3 kDa

ObservedMW: 40 kDa