Recombinant Human 4-1BB/TNFRSF9 Protein (Fc & His Tag)

SKU: PKSH032027

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: 4-1BB;TNFRSF9

Target Synonym: 4-1BB ligand receptor;CD137;CDw137;ILA;T-cell antigen 4-1BB homolog;T-cell antigen ILA;TNFRSF9

Research Areas: Cell biology;Cancer;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Fc-His

UNIProt ID: Q07011

Accession: Q07011

Background: Tumor necrosis factor receptor superfamily member 9(TNFRSF9) is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. It is absent from naive T cells; but upregulated and continually expressed following T cell activation. It is a receptor for TNFSF9/4-1BBL; and possibly active during T cell activation.

Concentration:

Activity: Immobilized Human 4-1BB-Fc-His at 2μg/ml (100 μl/well)can bind Anti-Human CD137 mAb .The ED₅₀ of Anti-Human CD137 mAb is 0.41ug/ml.

Sequence: Leu24-Gln186

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.0 kDa

ObservedMW: 55-75 kDa