GeneBio Systems
Recombinant HIV gp120 (C-8His)
Recombinant HIV gp120 (C-8His)
SKU:EPT033
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Size:10ug
Description:Recombinant HIV 1 Envelope Glycoprotein Gp120 is produced by our Mammalian expression system and the target gene encoding Met1-Glu498 is expressed with a 8His tag at the C-terminus.
Accession:Q9DKG6
Molecular weight:57 KDa
Apparent molecular weight:90-140 KDa, reducing conditions
Other names:Envelope glycoprotein gp120; Glycoprotein 120; Surface protein gp120
Storage condition:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Biological activity:
Redissolve:Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Background:Envelope glycoprotein gp160 is single-pass type I membrane protein. The mature envelope protein (Env) consists of a homotrimer of non-covalently associated gp120-gp41 heterodimers. It is cleaved into the following 2 chains: glycoprotein 120 and transmembrane protein gp41. The resulting complex protrudes from the virus surface as a spike. The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo.
