Rat TNFaIP6(Tumor Necrosis Factor Alpha Induced Protein 6) ELISA Kit

SKU: ELK10095

Size:96T

Reactivity:Rat

Alternative Names:TSG6; TNF-Stimulated Gene 6; Hyaluronate-binding protein; Tumor necrosis factor alpha-induced protein 6

Assay Type:Sandwich

Sensitivity:0.051 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFaIP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFaIP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFaIP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFaIP6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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