Rat E2(Estradiol) ELISA Kit

SKU: ELK10932

Size:96T

Reactivity:Rat

Alternative Names:17B-Estradiol; Oestradiol; Beta-Estradiol

Assay Type:Competitive Inhibition

Sensitivity:14.45 pg/mL

Standard:3000 pg/mL

Detection range:46.88-3000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Endocrinology;Reproductive science;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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