Rat DNA (cytosine-5)-methyltransferase 1,DNMT1 ELISA KIT

SKU: E3389Ra

Size:96 Tests-1 Kit

Background:Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth. {ECO:0000250|UniProtKB:P26358}.

Research_area:

Applications:ELISA

Target_protein:Dnmt1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.24ng/ml

Detection range:0.5-32ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9Z330

MASS(Da):182774

GeneID:

Synonyms:Dnmt1

Gene names:Dnmt1

Standard solution:64ng/ml