GeneBio Systems

RANkL ELISA kit (Rat)

Name: RANkL ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEA855Ra
Price: 974.95 EUR
Availability: InStock

SKU:SEA855Ra

€974,95 EUR

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Quantity

Size: 96Tests

# of Times Cited in literature: 20

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: RANkL

Target Full Name: Receptor Activator Of Nuclear Factor Kappa B Ligand

Alternative Names: CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine

Target Species: Rat

Uniprot: Q9ESE2

Gene ID: 117516

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat RANkL / Receptor Activator Of Nuclear Factor Kappa B Ligand

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 15.6-1,000pg/mL

Sensitivity: 5.7pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Metabolic pathway;Apoptosis;Tumor immunity;Bone metabolism;

References Citing This Product: The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model

Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells

Effect of Nicotine on RANKL and OPG and Bone Mineral Density

Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats

A novel model of bisphosphonate-related osteonecrosis of the jaw in rats

Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.

Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats.

The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats

Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity.

Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats

Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause