Rabbit Interleukin 10 (IL-10) ELISA Kit

SKU: AE38531RB

Size: 96T. Other sizes are also available.

Species Reactivity: Rabbit (Oryctolagus cuniculus)

UniProt: Q9TSJ4

Abbreviation: IL-10

Alternative Names: CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 14.5 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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