Purified Rabbit anti-Thymidine Kinase 1 Antibody

SKU: DL94446A

Size 1: 100 ul

Synonyms : Thymidine kinase cytosolic

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Thymidine Kinase 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Thymidine Kinase 1. The exact sequence is proprietary.

WB description: Western blot analysis of Thymidine Kinase 1 expression in THP1 (A), Jurkat (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Thymidine Kinase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Thymidine Kinase 1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)

Gene ID(Human): 7083

Gene ID (Mouse): 21877

Gene ID(Rat): 24834

SwissProt (Human): P04183

SwissProt (Mouse): P04184

SwissProt (Rat): P27158

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.