Purified Rabbit anti-TEAD2 Antibody

SKU: DL94322A

Size 1: 100 ul

Synonyms : TEF4; Transcriptional enhancer factor TEF-4; TEA domain family member 2; TEAD-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, ChIP

Background: Rabbit polyclonal antibody to TEAD2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TEAD2. The exact sequence is proprietary.

WB description: Western blot analysis of TEAD2 expression in HEK293 (A), RAW264.7 (B), H9C2 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of TEAD2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TEAD2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100), ChIP (1/100 - 1/500)

Gene ID(Human): 8463

Gene ID (Mouse): 21677

Gene ID(Rat):

SwissProt (Human): Q15562

SwissProt (Mouse): P48301

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.