Purified Rabbit anti-TCP1-delta Antibody

SKU: DL96270A

Size 1: 100 ul

Synonyms : CCTD; SRB; T-complex protein 1 subunit delta; TCP-1-delta; CCT-delta; Stimulator of TAR RNA-binding

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TCP1-delta

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human TCP1-delta

WB description: Western blot analysis of TCP1-delta expression in MCF7 (A), THP1 (B), mouse spleen (C) whole cell lysates.

IHC description: Immunohistochemical analysis of TCP1-delta staining in rat stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TCP1-delta staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 10575

Gene ID (Mouse): 12464

Gene ID(Rat): 29374

SwissProt (Human): P50991

SwissProt (Mouse): P80315

SwissProt (Rat): Q7TPB1

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.