Purified Rabbit anti-Stim1 Antibody

SKU: DL99764A

Size 1: 100 ul

Synonyms : GOK; Stromal interaction molecule 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH, IF/IC

Background: Rabbit polyclonal antibody to Stim1

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human Stim1. The exact sequence is proprietary.

WB description: Western blot analysis of Stim1 expression in HeLa (A), mouse brain (B), mouse heart (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Stim1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Stim1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 6786

Gene ID (Mouse): 20866

Gene ID(Rat): 361618

SwissProt (Human): Q13586

SwissProt (Mouse): P70302

SwissProt (Rat): P84903

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.