Purified Rabbit anti-STAT1 (pS727) Antibody

SKU: DL91109A

Size 1: 100 ul

Synonyms : Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, D, Mk, P, S

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to STAT1 (pS727)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human STAT1. The exact sequence is proprietary.

WB description: Western blot analysis of STAT3 expression in A549 (A), LO2 (B), SP2/0 (C), NIH3T3 (D), PC12 (E), H9C2 (F) whole cell lysates.

IHC description: Immunohistochemical analysis of STAT3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of STAT3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 6774

Gene ID (Mouse): 20848

Gene ID(Rat): 25125

SwissProt (Human): P40763

SwissProt (Mouse): P42227

SwissProt (Rat): P52631

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.