Purified Rabbit anti-SP1 (pT739) Antibody

SKU: DL93525A

Size 1: 100 ul

Synonyms : TSFP1; Transcription factor Sp1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R, B, Mk

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SP1 (pT739)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SP1 (pT739). The exact sequence is proprietary.

WB description: Western blot analysis of SP1 (pT739) expression in A549 (A), K562 (B), rat muscle (C) whole cell lysates.

IHC description: Immunohistochemical analysis of SP1 (pT739) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 6667

Gene ID (Mouse): 20683

Gene ID(Rat): 24790

SwissProt (Human): P08047

SwissProt (Mouse): O89090

SwissProt (Rat): Q01714

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.