Purified Rabbit anti-RBFOX3 Antibody

SKU: DL95875A

Size 1: 100 ul

Synonyms : RNA binding protein fox-1 homolog 3; Fox-1 homolog C

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RBFOX3

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RBFOX3

WB description: Western blot analysis of RBFOX3 expression in A549 (A), mouse testis (B) whole cell lysates.

IHC description: Immunohistochemical analysis of RBFOX3 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RBFOX3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 146713

Gene ID (Mouse): 52897

Gene ID(Rat):

SwissProt (Human): A6NFN3

SwissProt (Mouse): Q8BIF2

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.