Purified Rabbit anti-PSMA6 Antibody

SKU: DL95992A

Size 1: 100 ul

Synonyms : PROS27; Proteasome subunit alpha type-6; 27 kDa prosomal protein; PROS-27; p27K; Macropain iota chain; Multicatalytic endopeptidase complex iota chain; Proteasome iota chain

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PSMA6

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PSMA6

WB description: Western blot analysis of PSMA6 expression in HepG2 (A), Hela (B), mouse skeletal muscle (C), PC12 (D), COS7 (E) whole cell lysates.

IHC description: Immunohistochemical analysis of PSMA6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PSMA6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 5687

Gene ID (Mouse): 26443

Gene ID(Rat): 29673

SwissProt (Human): P60900

SwissProt (Mouse): Q9QUM9

SwissProt (Rat): P60901

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.