Purified Rabbit anti-PRDM14 Antibody

SKU: DL96092A

Size 1: 100 ul

Synonyms : PR domain zinc finger protein 14; PR domain-containing protein 14

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP, ChIP

Background: Rabbit polyclonal antibody to PRDM14

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PRDM14

WB description: Western blot analysis of PRDM14 expression in BT474 (A), Jurkat (B), Hela (C) whole cell lysates.

IHC description: Immunohistochemical analysis of PRDM14 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PRDM14 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/100), ChIP (1/20 - 1/100)

Gene ID(Human): 63978

Gene ID (Mouse): 383491

Gene ID(Rat):

SwissProt (Human): Q9GZV8

SwissProt (Mouse): E9Q3T6

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.