Purified Rabbit anti-PDC-E2 Antibody

SKU: DL96184A

Size 1: 100 ul

Synonyms : DLTA; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PBC; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to PDC-E2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PDC-E2

WB description: Western blot analysis of PDC-E2 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D), rat kidney (E) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of PDC-E2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Gene ID(Human): 1737

Gene ID (Mouse): 235339

Gene ID(Rat): 81654

SwissProt (Human): P10515

SwissProt (Mouse): Q8BMF4

SwissProt (Rat): P08461

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.