Purified Rabbit anti-PAK4/5 Antibody

SKU: DL98633A

Size 1: 100 ul

Synonyms : KIAA1142; Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IC

Background: Rabbit polyclonal antibody to PAK4/5

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PAK4/5. The exact sequence is proprietary.

WB description: Western blot analysis of PAK4/5 expression in C6 (A), CT26 (B), A2780 (C), HCT116 (D), PC3 (E) whole cell lysates.

IHC description:

IF/ICC description: Immunocytochemistry analysis of PAK4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IC (1/100 - 1/500)

Gene ID(Human): 10298

Gene ID (Mouse): 70584

Gene ID(Rat):

SwissProt (Human): O96013; Q9P286

SwissProt (Mouse): Q8BTW9

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.