Purified Rabbit anti-Nop30 Antibody

SKU: DL96200A

Size 1: 100 ul

Synonyms : ARC; NOP; Nucleolar protein 3; Apoptosis repressor with CARD; Muscle-enriched cytoplasmic protein; Myp; Nucleolar protein of 30 kDa; Nop30

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Nop30

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Nop30

WB description: Western blot analysis of Nop30 expression in BT474 (A), MCF7 (B), mouse heart (C), mouse lung (D), rat heart (E) whole cell lysates.

IHC description: Immunohistochemical analysis of Nop30 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Nop30 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Gene ID(Human): 8996

Gene ID (Mouse): 78688

Gene ID(Rat): 85383

SwissProt (Human): O60936

SwissProt (Mouse): Q9D1X0

SwissProt (Rat): Q62881

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.