Purified Rabbit anti-NF-kappaB p65 Antibody

SKU: DL91001A

Size 1: 100 ul

Synonyms : NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC

Background: Rabbit polyclonal antibody to NF-kappaB p65

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p65. The exact sequence is proprietary.

WB description: Western blot analysis of NF-kappaB p65 expression in DLD (A), mouse hert (B), rat brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of NF-kappaB p65 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5970

Gene ID (Mouse): 19697

Gene ID(Rat):

SwissProt (Human): Q04206

SwissProt (Mouse): Q04207

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.