Purified Rabbit anti-NF-kappaB p100 Antibody

SKU: DL93758A

Size 1: 100 ul

Synonyms : LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, P

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to NF-kappaB p100

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p100. The exact sequence is proprietary.

WB description: Western blot analysis of NF-kappaB p100 expression in Jurkat (A), MCF7 (B), HeLa (C) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of NF-kappaB p100 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Gene ID(Human): 4791

Gene ID (Mouse): 18034

Gene ID(Rat):

SwissProt (Human): Q00653

SwissProt (Mouse): Q9WTK5

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.