Purified Rabbit anti-NELF-E Antibody

SKU: DL96374A

Size 1: 100 ul

Synonyms : RD; RDBP; Negative elongation factor E; NELF-E; RNA-binding protein RD

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to NELF-E

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human NELF-E

WB description: Western blot analysis of NELF-E expression in SW620 (A), Hela (B), mouse liver (C), mouse testis (D) whole cell lysates.

IHC description: Immunohistochemical analysis of NELF-E staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of NELF-E staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 7936

Gene ID (Mouse): 27632

Gene ID(Rat):

SwissProt (Human): P18615

SwissProt (Mouse): P19426

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.