Purified Rabbit anti-NAT13 Antibody

SKU: DL96450A

Size 1: 100 ul

Synonyms : MAK3; NAT13; NAT5; N-alpha-acetyltransferase 50; N-acetyltransferase 13; N-acetyltransferase 5; hNAT5; N-acetyltransferase san homolog; hSAN; NatE catalytic subunit

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to NAT13

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human NAT13

WB description: Western blot analysis of NAT13 expression in SKOV3 (A), MCF7 (B), mouse brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of NAT13 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of NAT13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 80218

Gene ID (Mouse): 72117

Gene ID(Rat):

SwissProt (Human): Q9GZZ1

SwissProt (Mouse): Q6PGB6

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.