Purified Rabbit anti-Lamin A/C Antibody

SKU: DL90690A

Size 1: 100 ul

Synonyms : LMN1; Prelamin-A/C

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, P

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to Lamin A/C

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary.

WB description: Western blot analysis of SMAD4 expression in HepG2 (A), HeLa (B), NIH3T3 (C), COS7 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of SMAD4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of SMAD4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 4089

Gene ID (Mouse): 17128

Gene ID(Rat): 50554

SwissProt (Human): Q13485

SwissProt (Mouse): P97471

SwissProt (Rat): O70437

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.