Purified Rabbit anti-IL-21 Antibody

SKU: DL96895A

Size 1: 100 ul

Synonyms : Interleukin-21; IL-21; Za11

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to IL-21

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human IL-21

WB description: Western blot analysis of IL-21 expression in mouse testis (A), mouse liver (B), mouse skin (C) whole cell lysates.

IHC description: Immunohistochemical analysis of IL-21 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of IL-21 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 59067

Gene ID (Mouse):

Gene ID(Rat): 365769

SwissProt (Human): Q9HBE4

SwissProt (Mouse):

SwissProt (Rat): A3QPB9

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.