Purified Rabbit anti-IARS2 Antibody

SKU: DL91714A

Size 1: 100 ul

Synonyms : Isoleucine--tRNA ligase, mitochondrial; Isoleucyl-tRNA synthetase; IleRS

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to IARS2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IARS2. The exact sequence is proprietary.

WB description: Western blot analysis of IARS2 expression in Jurkat (A), HT1080 (B), NIH3T3 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of IARS2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of IARS2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 55699

Gene ID (Mouse): 381314

Gene ID(Rat):

SwissProt (Human): Q9NSE4

SwissProt (Mouse): Q8BIJ6

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.