Purified Rabbit anti-HSF1 Antibody

SKU: DL98827A

Size 1: 100 ul

Synonyms : HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, B

Applications: WB, IH

Background: Rabbit polyclonal antibody to HSF1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSF1. The exact sequence is proprietary.

WB description: Western blot analysis of HSF1 expression in MEF (A), A549 (B), Hela (C), MCF7 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of HSF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 3297

Gene ID (Mouse): 15499

Gene ID(Rat):

SwissProt (Human): Q00613

SwissProt (Mouse): P38532

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.