Purified Rabbit anti-Histone H4 (MonoMethyl-K5) Antibody

SKU: DL99045A

Size 1: 100 ul

Synonyms : H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, P

Applications: WB, IH

Background: Rabbit polyclonal antibody to Histone H4 (MonoMethyl-K5)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 with a site at MonoMethyl-K5. The exact sequence is proprietary.

WB description: Western blot analysis of Histone H4 (MonoMethyl-K5) expression in Hela (A) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone H4 (MonoMethyl-K5) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 121504; 554313; 8294; 8359; 8360; 8361; 8362; 8363; 8364; 8365; 8366; 8367; 8368; 8370

Gene ID (Mouse): 100041230; 102641229; 319155; 319156; 319157; 319158; 319159; 319160; 319161; 320332; 326619; 326620; 69386; 97122

Gene ID(Rat): 100360950; 100912290; 100912418; 100912564; 102548682; 102551184; 102557184; 291152; 295277; 500351; 502913; 64627; 680097

SwissProt (Human): P62805

SwissProt (Mouse): P62806

SwissProt (Rat): P62804

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.