Purified Rabbit anti-FIR Antibody

SKU: DL96315A

Size 1: 100 ul

Synonyms : FIR; ROBPI; SIAHBP1; Poly(U)-binding-splicing factor PUF60; 60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to FIR

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human FIR

WB description: Western blot analysis of FIR expression in A549 (A), mouse brain (B), mouse spleen (C), rat lung (D), rat liver (E) whole cell lysates.

IHC description: Immunohistochemical analysis of FIR staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of FIR staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 22827

Gene ID (Mouse): 67959

Gene ID(Rat):

SwissProt (Human): Q9UHX1

SwissProt (Mouse): Q3UEB3

SwissProt (Rat): Q9WV25

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.