Purified Rabbit anti-FIH-1 Antibody

SKU: DL96081A

Size 1: 100 ul

Synonyms : FIH1; Hypoxia-inducible factor 1-alpha inhibitor; Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP, ChIP

Background: Rabbit polyclonal antibody to FIH-1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human FIH-1

WB description: Western blot analysis of FIH-1 expression in MCF7 (A), K562 (B), mouse heart (C) whole cell lysates.

IHC description: Immunohistochemical analysis of FIH-1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of FIH-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/200), ChIP (1/20 - 1/100)

Gene ID(Human): 55662

Gene ID (Mouse): 319594

Gene ID(Rat):

SwissProt (Human): Q9NWT6

SwissProt (Mouse): Q8BLR9

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.