Purified Rabbit anti-EEF1D Antibody

SKU: DL96804A

Size 1: 100 ul

Synonyms : EF1D; Elongation factor 1-delta; EF-1-delta; Antigen NY-CO-4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to EEF1D

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human EEF1D

WB description: Western blot analysis of EEF1D expression in SHSY5Y (A), Jurkat (B), A549 (C), mouse spleen (D) whole cell lysates.

IHC description: Immunohistochemical analysis of EEF1D staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of EEF1D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 1936

Gene ID (Mouse): 66656

Gene ID(Rat): 300033

SwissProt (Human): P29692

SwissProt (Mouse): P57776

SwissProt (Rat): Q68FR9

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.