Purified Rabbit anti-DARPP32 (pT75) Antibody

SKU: DL93166A

Size 1: 100 ul

Synonyms : DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to DARPP32 (pT75)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DARPP32 (pT75). The exact sequence is proprietary.

WB description: Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A), SHSY5Y (B) whole cell lysates.

IHC description: Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 84152

Gene ID (Mouse): 19049

Gene ID(Rat): 360616

SwissProt (Human): Q9UD71

SwissProt (Mouse): Q60829

SwissProt (Rat): Q6J4I0

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.