Purified Rabbit anti-Cyclin E1 (pT395) Antibody

SKU: DL92161A

Size 1: 100 ul

Synonyms : CCNE; G1/S-specific cyclin-E1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Cyclin E1 (pT395)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin E1 (pT395). The exact sequence is proprietary.

WB description: Western blot analysis of Cyclin E1 (pT395) expression in HepG2 (A), JAR (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin E1 (pT395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 898

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P24864

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.