Purified Rabbit anti-CMPK1 Antibody

SKU: DL93604A

Size 1: 100 ul

Synonyms : CMK; CMPK; UCK; UMK; UMPK; UMP-CMP kinase; Deoxycytidylate kinase; CK; dCMP kinase; Nucleoside-diphosphate kinase; Uridine monophosphate/cytidine monophosphate kinase; UMP/CMP kinase; UMP/CMPK

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to CMPK1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CMPK1. The exact sequence is proprietary.

WB description: Western blot analysis of CMPK1 expression in HEK293T (A), K562 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of CMPK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of CMPK1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 51727

Gene ID (Mouse): 66588

Gene ID(Rat): 298410

SwissProt (Human): P30085

SwissProt (Mouse): Q9DBP5

SwissProt (Rat): Q4KM73

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.